Translational_Unit

Part:BBa_K4912012:Design

Designed by: Jiang Yunchong   Group: iGEM23_HUBU-HUBEI-CHINA   (2023-10-10)


Key enzymes for synthesizing hydroxytyrosol


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1093
    Illegal BglII site found at 869
    Illegal BglII site found at 2045
    Illegal BglII site found at 2370
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4150


Design Notes

Compared to part BBa_ K4912008, replace promoter P43 with PbacA and replace promoter PbacA with P43


Source

Bacillus licheniformis，E. coli，Lactococcus,bacillus subtilis

References